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Schizosaccharomyces pombe Postgenome Database

Gene name SPAC57A10.04
Gene ID 16/G02
Gene synonyms/obsolete
Gene product hypothetical protein; sequence orphan; low similarity to RhoGEF
Entry clone Cloned
ORF length (unspliced) 1080
ORF length (spliced) 1017
Entry clone length 1080
No. of intron 1
Sequence status Finished
Sequence results 184A:G
Comments
Polymerase used for cloning Platinum Taq HiFi (Invitrogen)
Fwd primer name SPAC57A10.04.Fd
Fwd primer SEQ AAAAAGCAGGCTCTCATATGCCAATCATAAGGTCTCT
Rev primer name SPAC57A10.04.Rv
Rev primer SEQ AGAAAGCTGGGTATGTTTTAGCATTGACAACT
Amino acid length 338
Molecular weight 38.4
Isoelectric point (calc.) 9.1
Signal SEQ
No. of transmembrane domain
NLS position (Columbia Univ. Bioinformatics Center) none
NES motif ( L-x(2,3)-[IVLMF]-x(2,3)-L-x-[LI]) none
Localization (YFP) cytoplasmic dots; nucleus
Comments for localization
Effect of LMB on protein localization no change
Microscope used for observation DeltaVision

Image information
YFP 4 images) See all images

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