Gene name |
SPAC57A10.04 |
Gene ID |
16/G02 |
Gene synonyms/obsolete |
|
Gene product |
hypothetical protein;
sequence orphan; low similarity to RhoGEF |
Entry clone |
Cloned |
ORF length (unspliced) |
1080 |
ORF length (spliced) |
1017 |
Entry clone length |
1080 |
No. of intron |
1 |
Sequence status |
Finished |
Sequence results |
184A:G |
Comments |
|
Polymerase used for cloning |
Platinum Taq HiFi
(Invitrogen) |
Fwd primer name |
SPAC57A10.04.Fd |
Fwd primer SEQ |
AAAAAGCAGGCTCTCATATGCCAATCATAAGGTCTCT |
Rev primer name |
SPAC57A10.04.Rv |
Rev primer SEQ |
AGAAAGCTGGGTATGTTTTAGCATTGACAACT |
Amino acid length |
338 |
Molecular weight |
38.4 |
Isoelectric point (calc.) |
9.1 |
Signal SEQ |
|
No. of transmembrane domain |
|
NLS position (Columbia Univ.
Bioinformatics Center) |
none |
NES motif (
L-x(2,3)-[IVLMF]-x(2,3)-L-x-[LI]) |
none |
Localization (YFP) |
cytoplasmic dots;
nucleus |
Comments for localization |
|
Effect of LMB on protein
localization |
no change |
Microscope used for
observation |
DeltaVision |