Gene name |
SPBC660.03c |
Gene ID |
16/H07 |
Gene synonyms/obsolete |
SPBC1198.13c |
Gene product |
transcription
initiation factor TFIIF (beta subunit) |
Entry clone |
Cloned |
ORF length (unspliced) |
1086 |
ORF length (spliced) |
924 |
Entry clone length |
1086 |
No. of intron |
2 |
Sequence status |
Finished |
Sequence results |
100% match |
Comments |
|
Polymerase used for cloning |
Platinum Taq HiFi
(Invitrogen) |
Fwd primer name |
SPBC660.03.Fd |
Fwd primer SEQ |
AAAAAGCAGGCTCTCATATGTCTGAAGAAAAACCGAC |
Rev primer name |
SPBC660.03.Rv |
Rev primer SEQ |
AGAAAGCTGGGTAAACAACATCAATCATCTCA |
Amino acid length |
307 |
Molecular weight |
34.6 |
Isoelectric point (calc.) |
4.8 |
Signal SEQ |
|
No. of transmembrane domain |
|
NLS position (Columbia Univ.
Bioinformatics Center) |
none |
NES motif (
L-x(2,3)-[IVLMF]-x(2,3)-L-x-[LI]) |
none |
Localization (YFP) |
SPB?; nucleus; nuclear
dots |
Comments for localization |
|
Effect of LMB on protein
localization |
no change |
Microscope used for
observation |
DeltaVision |