Gene name |
SPAC3G9.09c |
Gene ID |
17/A04 |
Gene synonyms/obsolete |
tif211 |
Gene product |
translation initiation
factor (2 alpha subunit) |
Entry clone |
Cloned |
ORF length (unspliced) |
1089 |
ORF length (spliced) |
921 |
Entry clone length |
1089 |
No. of intron |
2 |
Sequence status |
Finished |
Sequence results |
134T:C /
333T:addition |
Comments |
|
Polymerase used for cloning |
Platinum Taq HiFi
(Invitrogen) |
Fwd primer name |
SPAC3G9.09.Fd |
Fwd primer SEQ |
AAAAAGCAGGCTCTCATATGTCGACGACAAGCTGCAG |
Rev primer name |
SPAC3G9.09.Rv |
Rev primer SEQ |
AGAAAGCTGGGTACTCAGAACCGCTTTGGTCA |
Amino acid length |
306 |
Molecular weight |
34.5 |
Isoelectric point (calc.) |
6.4 |
Signal SEQ |
|
No. of transmembrane domain |
|
NLS position (Columbia Univ.
Bioinformatics Center) |
none |
NES motif (
L-x(2,3)-[IVLMF]-x(2,3)-L-x-[LI]) |
LTNALDKSLGL |
Localization (YFP) |
cytosol=nucleus |
Comments for localization |
|
Effect of LMB on protein
localization |
no change |
Microscope used for
observation |
Confocal
|