Gene name |
SPBC3B8.07c |
Gene ID |
17/A06 |
Gene synonyms/obsolete |
dsd1 |
Gene product |
dihydrosphingosine
delta 4 desaturase (pers. comm. Jonathan Napier); sphingolipid
delta-4-desaturase (dihydroceramide desaturase) (pers. comm.
Jonathan Napier); possible early signal for meiotic
differentiation; fatty acid desaturase; no apparent Sc
ortholog |
Entry clone |
Cloned |
ORF length (unspliced) |
1089 |
ORF length (spliced) |
|
Entry clone length |
1089 |
No. of intron |
0 |
Sequence status |
Finished |
Sequence results |
100% match |
Comments |
|
Polymerase used for cloning |
Platinum Taq HiFi
(Invitrogen) |
Fwd primer name |
SPBC3B8.07.Fd |
Fwd primer SEQ |
AAAAAGCAGGCTCTCATATGGCCGAATCAACTGCTAC |
Rev primer name |
SPBC3B8.07.Rv |
Rev primer SEQ |
AGAAAGCTGGGTATGAAACATGAAGGTGCATT |
Amino acid length |
362 |
Molecular weight |
41.8 |
Isoelectric point (calc.) |
7.8 |
Signal SEQ |
|
No. of transmembrane domain |
5 |
NLS position (Columbia Univ.
Bioinformatics Center) |
none |
NES motif (
L-x(2,3)-[IVLMF]-x(2,3)-L-x-[LI]) |
none |
Localization (YFP) |
ER |
Comments for localization |
|
Effect of LMB on protein
localization |
no change |
Microscope used for
observation |
Confocal
|