Gene name |
SPAC16.03c |
Gene ID |
17/A08 |
Gene synonyms/obsolete |
ura2 |
Gene product |
dihydroorotase;
involved in pyrimidine nucleotide biosynthesis; dihydroorotase
activity |
Entry clone |
Cloned |
ORF length (unspliced) |
1091 |
ORF length (spliced) |
1014 |
Entry clone length |
1091 |
No. of intron |
1 |
Sequence status |
Finished |
Sequence results |
100% match |
Comments |
|
Polymerase used for cloning |
Platinum Taq HiFi
(Invitrogen) |
Fwd primer name |
SPAC16.03.Fd |
Fwd primer SEQ |
AAAAAGCAGGCTCTCATATGAGTTTGAAAATACCTGG |
Rev primer name |
SPAC16.03.Rv |
Rev primer SEQ |
AGAAAGCTGGGTACTCCCATGAACAGTGCCAT |
Amino acid length |
337 |
Molecular weight |
37.5 |
Isoelectric point (calc.) |
6.6 |
Signal SEQ |
|
No. of transmembrane domain |
|
NLS position (Columbia Univ.
Bioinformatics Center) |
none |
NES motif (
L-x(2,3)-[IVLMF]-x(2,3)-L-x-[LI]) |
LHQRFPKLKI |
Localization (YFP) |
periphery |
Comments for localization |
|
Effect of LMB on protein
localization |
no change |
Microscope used for
observation |
Confocal
|