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Schizosaccharomyces pombe Postgenome Database

Gene name SPCC1183.06
Gene ID 17/B07
Gene synonyms/obsolete ung1
Gene product uracil DNA N-glycosylase; uracil DNA N-glycosylase activity; overexpression induces checkpoint-dependent cell cycle delay
Entry clone Cloned
ORF length (unspliced) 1093
ORF length (spliced) 969
Entry clone length 1093
No. of intron 2
Sequence status Finished
Sequence results 464T:C
Comments
Polymerase used for cloning Platinum Taq HiFi (Invitrogen)
Fwd primer name SPCC1183.06.Fd
Fwd primer SEQ AAAAAGCAGGCTCTCATATGACTGTTCTGAATACTAC
Rev primer name SPCC1183.06.Rv
Rev primer SEQ AGAAAGCTGGGTACTCAGTTGAAGAGGATTCC
Amino acid length 322
Molecular weight 36.7
Isoelectric point (calc.) 9.6
Signal SEQ
No. of transmembrane domain
NLS position (Columbia Univ. Bioinformatics Center) none
NES motif ( L-x(2,3)-[IVLMF]-x(2,3)-L-x-[LI]) none
Localization (YFP) no apparent signal
Comments for localization
Effect of LMB on protein localization not determined
Microscope used for observation Confocal

Image information
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