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Schizosaccharomyces pombe Postgenome Database

Gene name SPCC1281.08
Gene ID 17/C01
Gene synonyms/obsolete wtf11
Gene product wtf element
Entry clone Cloned in 2004 trial
ORF length (unspliced) 1098
ORF length (spliced) 795
Entry clone length 1098
No. of intron 4
Sequence status Finished
Sequence results 100% match
Comments
Polymerase used for cloning Pyrobest DNA Pol (TaKaRa)
Fwd primer name SPCC1281.08.Fd
Fwd primer SEQ AAAAAGCAGGCTCTCATATGAACAGCAATTACGTTCC
Rev primer name SPCC1281.08.Rv
Rev primer SEQ AGAAAGCTGGGTACAATTCATCTGAATCTTCA
Amino acid length 264
Molecular weight 29.9
Isoelectric point (calc.) 4.9
Signal SEQ
No. of transmembrane domain 4
NLS position (Columbia Univ. Bioinformatics Center) none
NES motif ( L-x(2,3)-[IVLMF]-x(2,3)-L-x-[LI]) none
Localization (YFP) a few cytoplasmic dots; Golgi?
Comments for localization Golgi?; moving small cytoplasmic dots by over expression
Effect of LMB on protein localization no change
Microscope used for observation DeltaVision

Image information
YFP 3 images) See all images

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