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Schizosaccharomyces pombe Postgenome Database

Gene name SPAC1002.08c
Gene ID 17/C03
Gene synonyms/obsolete
Gene product RNA polymerase specificity factor
Entry clone Cloned
ORF length (unspliced) 1101
ORF length (spliced)
Entry clone length 1101
No. of intron 0
Sequence status Finished
Sequence results 113T:G / 401A:G
Comments
Polymerase used for cloning Platinum Taq HiFi (Invitrogen)
Fwd primer name SPAC1002.08.Fd
Fwd primer SEQ AAAAAGCAGGCTCTCATATGAAGCTTCCTAAAATTTT
Rev primer name SPAC1002.08.Rv
Rev primer SEQ AGAAAGCTGGGTATGAATCTTCAATTATTGTA
Amino acid length 366
Molecular weight 41.1
Isoelectric point (calc.) 8.7
Signal SEQ
No. of transmembrane domain
NLS position (Columbia Univ. Bioinformatics Center) none
NES motif ( L-x(2,3)-[IVLMF]-x(2,3)-L-x-[LI]) none
Localization (YFP) mitochondrion
Comments for localization
Effect of LMB on protein localization no change
Microscope used for observation DeltaVision

Image information
YFP 1 images) See all images

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