Gene name |
SPBC2G5.01 |
Gene ID |
17/F11 |
Gene synonyms/obsolete |
|
Gene product |
conserved
hypothetical; hypothetical protein |
Entry clone |
Cloned |
ORF length (unspliced) |
1125 |
ORF length (spliced) |
|
Entry clone length |
1125 |
No. of intron |
0 |
Sequence status |
Finished |
Sequence results |
374T:C / 616T:C /
702T:C |
Comments |
|
Polymerase used for cloning |
Platinum Taq HiFi
(Invitrogen) |
Fwd primer name |
SPBC2G5.01.Fd |
Fwd primer SEQ |
AAAAAGCAGGCTCTCATATGATTAATAAAAAGCTTTT |
Rev primer name |
SPBC2G5.01.Rv |
Rev primer SEQ |
AGAAAGCTGGGTACATCTTCTTTGCGCGTCTT |
Amino acid length |
374 |
Molecular weight |
43 |
Isoelectric point (calc.) |
9.4 |
Signal SEQ |
Predicted
(N-terminus) |
No. of transmembrane domain |
1 |
NLS position (Columbia Univ.
Bioinformatics Center) |
364 |
NES motif (
L-x(2,3)-[IVLMF]-x(2,3)-L-x-[LI]) |
none |
Localization (YFP) |
ER |
Comments for localization |
|
Effect of LMB on protein
localization |
no change |
Microscope used for
observation |
DeltaVision |