Chemical Genetics Laboratory
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Schizosaccharomyces pombe Postgenome Database

Gene name SPAC22A12.03c
Gene ID 17/G10
Gene synonyms/obsolete csn4
Gene product experimentally characterisedCOP9/signalosome complex (subunit 4); PCI domain; no apparent Sc ortholog; non-essential
Entry clone Cloned
ORF length (unspliced) 1134
ORF length (spliced)
Entry clone length 1134
No. of intron 0
Sequence status Finished
Sequence results 100% match
Comments
Polymerase used for cloning Platinum Taq HiFi (Invitrogen)
Fwd primer name SPAC22A12.03.Fd
Fwd primer SEQ AAAAAGCAGGCTCTCATATGGAAGAAGTAGTTCATTA
Rev primer name SPAC22A12.03.Rv
Rev primer SEQ AGAAAGCTGGGTAAGAACCGTAATGTTGAAGA
Amino acid length 377
Molecular weight 43
Isoelectric point (calc.) 4.8
Signal SEQ
No. of transmembrane domain
NLS position (Columbia Univ. Bioinformatics Center) none
NES motif ( L-x(2,3)-[IVLMF]-x(2,3)-L-x-[LI]) LEQLLLLRI/LCSVAQCLLL
Localization (YFP) nucleus>>cytosol
Comments for localization
Effect of LMB on protein localization no change
Microscope used for observation Confocal

Image information
YFP 1 images) See all images

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