Gene name |
SPAC4G9.15 |
Gene ID |
17/H02 |
Gene synonyms/obsolete |
|
Gene product |
ketoreductase
activity; involved in fatty acid biosynthesis; involved in
fatty acid elongation; involved in very-long-chain fatty acid
metabolism |
Entry clone |
Cloned |
ORF length (unspliced) |
1135 |
ORF length (spliced) |
1026 |
Entry clone length |
1135 |
No. of intron |
2 |
Sequence status |
Finished |
Sequence results |
100% match |
Comments |
|
Polymerase used for cloning |
Platinum Taq HiFi
(Invitrogen) |
Fwd primer name |
SPAC4G9.15.Fd |
Fwd primer SEQ |
AAAAAGCAGGCTCTCATATGGACGGAGAAGGTATATA |
Rev primer name |
SPAC4G9.15.Rv |
Rev primer SEQ |
AGAAAGCTGGGTAGGCTTGATTTTGAGCTTGA |
Amino acid length |
341 |
Molecular weight |
37.3 |
Isoelectric point (calc.) |
10.1 |
Signal SEQ |
Predicted
(N-terminus) |
No. of transmembrane domain |
1 |
NLS position (Columbia Univ.
Bioinformatics Center) |
none |
NES motif (
L-x(2,3)-[IVLMF]-x(2,3)-L-x-[LI]) |
none |
Localization (YFP) |
ER |
Comments for localization |
accumulated ER by over
expression |
Effect of LMB on protein
localization |
no change |
Microscope used for
observation |
DeltaVision |