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Schizosaccharomyces pombe Postgenome Database

Gene name SPCC306.07c
Gene ID 17/H06
Gene synonyms/obsolete
Gene product conserved hypothetical protein
Entry clone Cloned
ORF length (unspliced) 1136
ORF length (spliced) 855
Entry clone length 1136
No. of intron 2
Sequence status Finished
Sequence results 100% match
Comments
Polymerase used for cloning Platinum Taq HiFi (Invitrogen)
Fwd primer name SPCC306.07.Fd
Fwd primer SEQ AAAAAGCAGGCTCTCATATGGCGATTACAAGTGAAAA
Rev primer name SPCC306.07.Rv
Rev primer SEQ AGAAAGCTGGGTACTTATTCCCTTTGCCTTCT
Amino acid length 284
Molecular weight 32.4
Isoelectric point (calc.) 10.4
Signal SEQ
No. of transmembrane domain
NLS position (Columbia Univ. Bioinformatics Center) none
NES motif ( L-x(2,3)-[IVLMF]-x(2,3)-L-x-[LI]) LDEVITKVLSI
Localization (YFP) nucleolus>>nucleus
Comments for localization
Effect of LMB on protein localization no change
Microscope used for observation Confocal

Image information
YFP 2 images) See all images

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