Chemical Genetics Laboratory
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Schizosaccharomyces pombe Postgenome Database

Gene name SPBC16E9.05
Gene ID 17/H08
Gene synonyms/obsolete
Gene product delta-sterol c-methyltransferase; involved in ergosterol biosynthesis
Entry clone Cloned
ORF length (unspliced) 1137
ORF length (spliced)
Entry clone length 1137
No. of intron 0
Sequence status Finished
Sequence results 720T:C
Comments
Polymerase used for cloning Platinum Taq HiFi (Invitrogen)
Fwd primer name SPBC16E9.05.Fd
Fwd primer SEQ AAAAAGCAGGCTCTCATATGAGTTCTACTGCCCTTCT
Rev primer name SPBC16E9.05.Rv
Rev primer SEQ AGAAAGCTGGGTATGCGTCCGTTTCGGGTTTC
Amino acid length 378
Molecular weight 42.8
Isoelectric point (calc.) 5.6
Signal SEQ
No. of transmembrane domain
NLS position (Columbia Univ. Bioinformatics Center) none
NES motif ( L-x(2,3)-[IVLMF]-x(2,3)-L-x-[LI]) none
Localization (YFP) nucleus; SPB?
Comments for localization nuclear dots by over expression?
Effect of LMB on protein localization no change
Microscope used for observation Confocal; DeltaVision

Image information
YFP 4 images) See all images

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