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Schizosaccharomyces pombe Postgenome Database

Gene name SPBC2D10.11c
Gene ID 18/A02
Gene synonyms/obsolete
Gene product involved in nucleosome assembly; similar to Sp SPCC364.06 (paralog)
Entry clone Cloned
ORF length (unspliced) 1140
ORF length (spliced)
Entry clone length 1140
No. of intron 0
Sequence status Finished
Sequence results 100% match
Comments
Polymerase used for cloning Platinum Taq HiFi (Invitrogen)
Fwd primer name SPBC2D10.11.Fd
Fwd primer SEQ AAAAAGCAGGCTCTCATATGTCTAAAGGTCCAGGAGA
Rev primer name SPBC2D10.11.Rv
Rev primer SEQ AGAAAGCTGGGTAATCACTAATTTCGCTACTC
Amino acid length 379
Molecular weight 43.3
Isoelectric point (calc.) 4.1
Signal SEQ
No. of transmembrane domain
NLS position (Columbia Univ. Bioinformatics Center) none
NES motif ( L-x(2,3)-[IVLMF]-x(2,3)-L-x-[LI]) none
Localization (YFP) cytosol=nucleus; cytoplasmic dots by over expression
Comments for localization
Effect of LMB on protein localization changed to: nucleus; one nuclear dot
Microscope used for observation Confocal

Image information
YFP 2 images) See all images
LMB 4 images) See all images

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