| Gene name |
SPCC830.07c |
| Gene ID |
18/A04 |
| Gene synonyms/obsolete |
psi1; psi |
| Gene product |
Status DNAJ domain
protein; involved in nuclear migration (sensu Fungi) (mitosis)
(required); involved in translation initiation |
| Entry clone |
Cloned |
| ORF length (unspliced) |
1140 |
| ORF length (spliced) |
|
| Entry clone length |
1140 |
| No. of intron |
0 |
| Sequence status |
Finished |
| Sequence results |
163T:C / 561T:C |
| Comments |
|
| Polymerase used for cloning |
Platinum Taq HiFi
(Invitrogen) |
| Fwd primer name |
SPCC830.07.Fd |
| Fwd primer SEQ |
AAAAAGCAGGCTCTCATATGGTAGCAGATACCAAATT |
| Rev primer name |
SPCC830.07.Rv |
| Rev primer SEQ |
AGAAAGCTGGGTAAAAGGCTTCTGCAGCAGTC |
| Amino acid length |
379 |
| Molecular weight |
40.2 |
| Isoelectric point (calc.) |
8.9 |
| Signal SEQ |
|
| No. of transmembrane domain |
|
| NLS position (Columbia Univ.
Bioinformatics Center) |
none |
| NES motif (
L-x(2,3)-[IVLMF]-x(2,3)-L-x-[LI]) |
none |
| Localization (YFP) |
cytosol=nucleus |
| Comments for localization |
|
| Effect of LMB on protein
localization |
no change |
| Microscope used for
observation |
Confocal |