| Gene name |
SPAC22A12.14c |
| Gene ID |
18/A08 |
| Gene synonyms/obsolete |
|
| Gene product |
transcriptional
regulator; involved in transcriptional regulation; BSD
domain |
| Entry clone |
Cloned |
| ORF length (unspliced) |
1143 |
| ORF length (spliced) |
1044 |
| Entry clone length |
1143 |
| No. of intron |
2 |
| Sequence status |
Finished |
| Sequence results |
778G:C |
| Comments |
|
| Polymerase used for cloning |
Platinum Taq HiFi
(Invitrogen) |
| Fwd primer name |
SPAC22A12.14.Fd |
| Fwd primer SEQ |
AAAAAGCAGGCTCTCATATGGATTTGTACGATTATAT |
| Rev primer name |
SPAC22A12.14.Rv |
| Rev primer SEQ |
AGAAAGCTGGGTATTCCCAGTCATCATCGTCT |
| Amino acid length |
347 |
| Molecular weight |
39.3 |
| Isoelectric point (calc.) |
3.8 |
| Signal SEQ |
|
| No. of transmembrane domain |
|
| NLS position (Columbia Univ.
Bioinformatics Center) |
none |
| NES motif (
L-x(2,3)-[IVLMF]-x(2,3)-L-x-[LI]) |
LESAVSKGLQI |
| Localization (YFP) |
cytosol; filaments;
cytoplasmic dots |
| Comments for localization |
aggregates at site of
septum formation by over expression |
| Effect of LMB on protein
localization |
no change |
| Microscope used for
observation |
DeltaVision |