Chemical Genetics Laboratory
PREVIOUS  NEXT
Schizosaccharomyces pombe Postgenome Database

Gene name SPBC428.02c
Gene ID 18/A10
Gene synonyms/obsolete eca39; SPBC582.12c
Gene product branched chain amino acid aminotransferase; phosphate dependent; involved in leucine biosynthesis; involved in valine biosynthesis; involved in isoleucine biosynthesis; non-essential
Entry clone Cloned
ORF length (unspliced) 1143
ORF length (spliced)
Entry clone length 1143
No. of intron 0
Sequence status Finished
Sequence results 100% match
Comments
Polymerase used for cloning Platinum Taq HiFi (Invitrogen)
Fwd primer name SPBC428.02.Fd
Fwd primer SEQ AAAAAGCAGGCTCTCATATGGTTCAAACTGCTGCTCT
Rev primer name SPBC428.02.Rv
Rev primer SEQ AGAAAGCTGGGTAAGGCAAAGCAGGAACGCTC
Amino acid length 380
Molecular weight 42.5
Isoelectric point (calc.) 7.8
Signal SEQ
No. of transmembrane domain
NLS position (Columbia Univ. Bioinformatics Center) none
NES motif ( L-x(2,3)-[IVLMF]-x(2,3)-L-x-[LI]) none
Localization (YFP) cytosol=nucleus
Comments for localization
Effect of LMB on protein localization no change
Microscope used for observation Confocal

Image information
YFP 1 images) See all images

For plasmid request Click!
Copyright (c) RIKEN (The Institute of Physical and Chemical Research), Japan. All rights reserved.