| Gene name |
SPBC3B9.02c |
| Gene ID |
18/B03 |
| Gene synonyms/obsolete |
cwf28;
SPBC3B9.02a |
| Gene product |
G-patch domain;
complexed with Cdc5p |
| Entry clone |
Cloned |
| ORF length (unspliced) |
1146 |
| ORF length (spliced) |
|
| Entry clone length |
1146 |
| No. of intron |
0 |
| Sequence status |
Finished |
| Sequence results |
301T:C / 940A:G |
| Comments |
|
| Polymerase used for cloning |
Platinum Taq HiFi
(Invitrogen) |
| Fwd primer name |
SPBC3B9.02a.Fd |
| Fwd primer SEQ |
AAAAAGCAGGCTCTCATATGAAGCGAAAAGCCGTATT |
| Rev primer name |
SPBC3B9.02a.Rv |
| Rev primer SEQ |
AGAAAGCTGGGTAAAGTGTACGAGTACTTCTG |
| Amino acid length |
381 |
| Molecular weight |
44.2 |
| Isoelectric point (calc.) |
8.9 |
| Signal SEQ |
|
| No. of transmembrane domain |
|
| NLS position (Columbia Univ.
Bioinformatics Center) |
67/355 |
| NES motif (
L-x(2,3)-[IVLMF]-x(2,3)-L-x-[LI]) |
none |
| Localization (YFP) |
SPB?; nucleus; spindle
microtubules |
| Comments for localization |
|
| Effect of LMB on protein
localization |
no change |
| Microscope used for
observation |
Confocal,
DeltaVision |