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Schizosaccharomyces pombe Postgenome Database

Gene name SPBC30B4.09c
Gene ID 18/B07
Gene synonyms/obsolete SPBC27B12.01c; SPBC30B4.09a
Gene product mitochondrial outer membrane protein; involved in mitochondrial biogenesis; involved in coupling mitochondria to the actin cytoskeleton
Entry clone Cloned
ORF length (unspliced) 1149
ORF length (spliced) 1041
Entry clone length 1149
No. of intron 3
Sequence status Finished
Sequence results 100% match
Comments
Polymerase used for cloning Platinum Taq HiFi (Invitrogen)
Fwd primer name SPBC30B4.09a.Fd
Fwd primer SEQ AAAAAGCAGGCTCTCATATGATACACCTGCCGCAGGG
Rev primer name SPBC30B4.09a.Rv
Rev primer SEQ AGAAAGCTGGGTAATCTTCATTAGATGACTCC
Amino acid length 346
Molecular weight 38.8
Isoelectric point (calc.) 8.3
Signal SEQ Predicted (N-terminus)
No. of transmembrane domain 1
NLS position (Columbia Univ. Bioinformatics Center) none
NES motif ( L-x(2,3)-[IVLMF]-x(2,3)-L-x-[LI]) none
Localization (YFP) ER; cytoplasmic dots
Comments for localization aggregates by over expression
Effect of LMB on protein localization no change
Microscope used for observation DeltaVision

Image information
YFP 2 images) See all images

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