| Gene name |
SPAC4A8.03c |
| Gene ID |
18/B10 |
| Gene synonyms/obsolete |
ptc4 |
| Gene product |
protein phosphatase
(2c isoform); involved in regulation of vacuole fusion;
non-essential; no apparent Sc ortholog; most closely related
to Sp Ptc1p |
| Entry clone |
Cloned |
| ORF length (unspliced) |
1152 |
| ORF length (spliced) |
|
| Entry clone length |
1152 |
| No. of intron |
0 |
| Sequence status |
Finished |
| Sequence results |
664T:C / 1061T:C |
| Comments |
|
| Polymerase used for cloning |
Platinum Taq HiFi
(Invitrogen) |
| Fwd primer name |
SPAC4A8.03.Fd |
| Fwd primer SEQ |
AAAAAGCAGGCTCTCATATGTCGATCCGTTTTCTTAA |
| Rev primer name |
SPAC4A8.03.Rv |
| Rev primer SEQ |
AGAAAGCTGGGTAACTTCTTCTGGGATGATAA |
| Amino acid length |
383 |
| Molecular weight |
43.5 |
| Isoelectric point (calc.) |
9 |
| Signal SEQ |
|
| No. of transmembrane domain |
|
| NLS position (Columbia Univ.
Bioinformatics Center) |
none |
| NES motif (
L-x(2,3)-[IVLMF]-x(2,3)-L-x-[LI]) |
LKRLRAPLYI |
| Localization (YFP) |
cytoplasmic dots |
| Comments for localization |
|
| Effect of LMB on protein
localization |
no change |
| Microscope used for
observation |
DeltaVision |