Gene name |
SPBC12C2.04 |
Gene ID |
18/C03 |
Gene synonyms/obsolete |
|
Gene product |
conserved
hypothetical; dehydrogenase; conserved in bacteria; no
apparent Sc ortholog; similar to Sp SPACUNK4.17
(paralog) |
Entry clone |
Cloned |
ORF length (unspliced) |
1155 |
ORF length (spliced) |
|
Entry clone length |
1155 |
No. of intron |
0 |
Sequence status |
Finished |
Sequence results |
100% match |
Comments |
|
Polymerase used for cloning |
Platinum Taq HiFi
(Invitrogen) |
Fwd primer name |
SPBC12C2.04.Fd |
Fwd primer SEQ |
AAAAAGCAGGCTCTCATATGACTGCTCTATCAACCAA |
Rev primer name |
SPBC12C2.04.Rv |
Rev primer SEQ |
AGAAAGCTGGGTAATTATTCGTAATGATCTTC |
Amino acid length |
384 |
Molecular weight |
43 |
Isoelectric point (calc.) |
5.8 |
Signal SEQ |
|
No. of transmembrane domain |
|
NLS position (Columbia Univ.
Bioinformatics Center) |
none |
NES motif (
L-x(2,3)-[IVLMF]-x(2,3)-L-x-[LI]) |
none |
Localization (YFP) |
nucleus>cytosol |
Comments for localization |
|
Effect of LMB on protein
localization |
no change |
Microscope used for
observation |
Confocal
|