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Schizosaccharomyces pombe Postgenome Database

Gene name SPCC320.13c
Gene ID 18/C05
Gene synonyms/obsolete ark1; SPCC330.16
Gene product serine/threonine protein kinase; aurora homolog; involved in spindle assembly checkpoint; involved in spindle formation (required); involved in sister chromatid separation (required); involved in microtubule attachment (required); interacts physically and/or genetically with the inner centromere protein Pic1p; interacts physically and/or genetically with the inner centromere protein Bir1p; interacts physically and/or genetically with survivin; involved in protein amino acid phosphorylation (SER10 of Hht3p)
Entry clone Cloned
ORF length (unspliced) 1155
ORF length (spliced)
Entry clone length 1155
No. of intron 0
Sequence status Finished
Sequence results 100% match
Comments
Polymerase used for cloning Platinum Taq HiFi (Invitrogen)
Fwd primer name SPCC330.16.Fd
Fwd primer SEQ AAAAAGCAGGCTCTCATATGGTGTTACCTCAAAATGT
Rev primer name SPCC330.16.Rv
Rev primer SEQ AGAAAGCTGGGTAGGAAGATTCAGAACTTTTG
Amino acid length 384
Molecular weight 43.8
Isoelectric point (calc.) 10.3
Signal SEQ
No. of transmembrane domain
NLS position (Columbia Univ. Bioinformatics Center) none
NES motif ( L-x(2,3)-[IVLMF]-x(2,3)-L-x-[LI]) none
Localization (YFP) no apparent signal
Comments for localization
Effect of LMB on protein localization not determined
Microscope used for observation Confocal

Image information
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