Gene name |
SPBC16E9.14c |
Gene ID |
18/C12 |
Gene synonyms/obsolete |
|
Gene product |
cation efflux family;
zinc-regulated; implicated in zinc-uptake |
Entry clone |
Cloned |
ORF length (unspliced) |
1161 |
ORF length (spliced) |
|
Entry clone length |
1161 |
No. of intron |
0 |
Sequence status |
Finished |
Sequence results |
422A:G / 778A:G |
Comments |
|
Polymerase used for cloning |
Platinum Taq HiFi
(Invitrogen) |
Fwd primer name |
SPBC16E9.14.Fd |
Fwd primer SEQ |
AAAAAGCAGGCTCTCATATGACTCAAAATCATAACAT |
Rev primer name |
SPBC16E9.14.Rv |
Rev primer SEQ |
AGAAAGCTGGGTAGGCTCTCTGGATATCTACT |
Amino acid length |
386 |
Molecular weight |
43 |
Isoelectric point (calc.) |
7 |
Signal SEQ |
|
No. of transmembrane domain |
6 |
NLS position (Columbia Univ.
Bioinformatics Center) |
none |
NES motif (
L-x(2,3)-[IVLMF]-x(2,3)-L-x-[LI]) |
LNSRFFHGLTL/LILVLLLSL/LSHLLSLTI |
Localization (YFP) |
cytosol=nucleus |
Comments for localization |
|
Effect of LMB on protein
localization |
no change |
Microscope used for
observation |
Confocal
|