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Schizosaccharomyces pombe Postgenome Database

Gene name SPAC3H1.13
Gene ID 18/D08
Gene synonyms/obsolete
Gene product serine/threonine protein kinase
Entry clone Cloned
ORF length (unspliced) 1166
ORF length (spliced) 1035
Entry clone length 1166
No. of intron 3
Sequence status Finished
Sequence results 886A:G
Comments
Polymerase used for cloning Platinum Taq HiFi (Invitrogen)
Fwd primer name SPAC3H1.13.Fd
Fwd primer SEQ AAAAAGCAGGCTCTCATATGCAAAATCTTTGGAAGAA
Rev primer name SPAC3H1.13.Rv
Rev primer SEQ AGAAAGCTGGGTATTGATTAATTTGCAAATCA
Amino acid length 344
Molecular weight 39
Isoelectric point (calc.) 7.1
Signal SEQ
No. of transmembrane domain
NLS position (Columbia Univ. Bioinformatics Center) none
NES motif ( L-x(2,3)-[IVLMF]-x(2,3)-L-x-[LI]) none
Localization (YFP) Golgi; ER?
Comments for localization
Effect of LMB on protein localization no change
Microscope used for observation DeltaVision

Image information
YFP 2 images) See all images

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