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Schizosaccharomyces pombe Postgenome Database

Gene name SPAC16E8.16
Gene ID 18/D10
Gene synonyms/obsolete
Gene product transcription initiation factor TFIIB
Entry clone Cloned
ORF length (unspliced) 1167
ORF length (spliced) 1023
Entry clone length 1167
No. of intron 1
Sequence status Finished
Sequence results 77A:G
Comments
Polymerase used for cloning Platinum Taq HiFi (Invitrogen)
Fwd primer name SPAC16E8.16.Fd
Fwd primer SEQ AAAAAGCAGGCTCTCATATGAACGCACCATCATTTAC
Rev primer name SPAC16E8.16.Rv
Rev primer SEQ AGAAAGCTGGGTAAGGCTTTGGTAACATAGCA
Amino acid length 340
Molecular weight 36.6
Isoelectric point (calc.) 8.7
Signal SEQ
No. of transmembrane domain
NLS position (Columbia Univ. Bioinformatics Center) none
NES motif ( L-x(2,3)-[IVLMF]-x(2,3)-L-x-[LI]) LMVRFCNRLML
Localization (YFP) nucleus
Comments for localization
Effect of LMB on protein localization no change
Microscope used for observation Confocal

Image information
YFP 2 images) See all images

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