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Schizosaccharomyces pombe Postgenome Database

Gene name SPAC328.09
Gene ID 18/E03
Gene synonyms/obsolete
Gene product mitochondrial carrier
Entry clone Cloned
ORF length (unspliced) 1168
ORF length (spliced) 897
Entry clone length 1168
No. of intron 1
Sequence status Finished
Sequence results 326T:C
Comments
Polymerase used for cloning Platinum Taq HiFi (Invitrogen)
Fwd primer name SPAC328.09.Fd
Fwd primer SEQ AAAAAGCAGGCTCTCATATGCCTCATGATAATATTCC
Rev primer name SPAC328.09.Rv
Rev primer SEQ AGAAAGCTGGGTAAGCAGAGGCGTTATGTACA
Amino acid length 298
Molecular weight 33
Isoelectric point (calc.) 10.4
Signal SEQ
No. of transmembrane domain
NLS position (Columbia Univ. Bioinformatics Center) none
NES motif ( L-x(2,3)-[IVLMF]-x(2,3)-L-x-[LI]) none
Localization (YFP) ambiguous structure
Comments for localization
Effect of LMB on protein localization no change
Microscope used for observation Confocal

Image information
YFP 2 images) See all images

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