| Gene name |
SPAC27E2.07 |
| Gene ID |
18/E06 |
| Gene synonyms/obsolete |
|
| Gene product |
glycosyltransferase;
involved in cell wall biosynthesis; deletion mutant
galactomannans deficient for pyruvylated
galactose-beta-1,3-galactose-alpha-1,2-pyruvylgalactose
(PvGal) (pers. comm. Robert Trimble); no apparent
orthologs |
| Entry clone |
Cloned |
| ORF length (unspliced) |
1170 |
| ORF length (spliced) |
|
| Entry clone length |
1170 |
| No. of intron |
0 |
| Sequence status |
Finished |
| Sequence results |
100% match |
| Comments |
|
| Polymerase used for cloning |
Platinum Taq HiFi
(Invitrogen) |
| Fwd primer name |
SPAC27E2.07.Fd |
| Fwd primer SEQ |
AAAAAGCAGGCTCTCATATGACTAAACTCTGGGTAAA |
| Rev primer name |
SPAC27E2.07.Rv |
| Rev primer SEQ |
AGAAAGCTGGGTAAGCAGTTATCAAGTCGAAT |
| Amino acid length |
389 |
| Molecular weight |
45 |
| Isoelectric point (calc.) |
5.6 |
| Signal SEQ |
Predicted
(N-terminus) |
| No. of transmembrane domain |
1 |
| NLS position (Columbia Univ.
Bioinformatics Center) |
none |
| NES motif (
L-x(2,3)-[IVLMF]-x(2,3)-L-x-[LI]) |
none |
| Localization (YFP) |
cytosol=nucleus |
| Comments for localization |
|
| Effect of LMB on protein
localization |
no change |
| Microscope used for
observation |
Confocal
|