Gene name |
SPAC24B11.08c |
Gene ID |
18/E10 |
Gene synonyms/obsolete |
|
Gene product |
COPII-coated vesicle
component; involved in intracellular protein transport;
involved in ER to Golgi transport; predicted N-terminal signal
sequence |
Entry clone |
Cloned |
ORF length (unspliced) |
1173 |
ORF length (spliced) |
|
Entry clone length |
1173 |
No. of intron |
0 |
Sequence status |
Finished |
Sequence results |
170A:T / 550A:G /
884A:G |
Comments |
|
Polymerase used for cloning |
Platinum Taq HiFi
(Invitrogen) |
Fwd primer name |
SPAC24B11.08.Fd |
Fwd primer SEQ |
AAAAAGCAGGCTCTCATATGCAATTCCGTTCACCCCT |
Rev primer name |
SPAC24B11.08.Rv |
Rev primer SEQ |
AGAAAGCTGGGTAATTTTTCTTTAACTTTTGA |
Amino acid length |
390 |
Molecular weight |
43.7 |
Isoelectric point (calc.) |
6.5 |
Signal SEQ |
Predicted
(N-terminus) |
No. of transmembrane domain |
2 |
NLS position (Columbia Univ.
Bioinformatics Center) |
none |
NES motif (
L-x(2,3)-[IVLMF]-x(2,3)-L-x-[LI]) |
none |
Localization (YFP) |
ER |
Comments for localization |
|
Effect of LMB on protein
localization |
no change |
Microscope used for
observation |
Confocal
|