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Schizosaccharomyces pombe Postgenome Database

Gene name SPBC19C7.12c
Gene ID 18/F01
Gene synonyms/obsolete
Gene product alpha-1,2-mannosyltransferase; glycosyl transferase family 15; similar to S. cerevisiae YOR099W and YKR061W and YJL139C and YDR483W and YBR205W and YBR199W and YPL053C; conserved fungal protein
Entry clone Cloned
ORF length (unspliced) 1173
ORF length (spliced)
Entry clone length 1173
No. of intron 0
Sequence status Finished
Sequence results 100% match
Comments
Polymerase used for cloning Platinum Taq HiFi (Invitrogen)
Fwd primer name SPBC19C7.12.Fd
Fwd primer SEQ AAAAAGCAGGCTCTCATATGGTTCGCTTGCCAAGAAA
Rev primer name SPBC19C7.12.Rv
Rev primer SEQ AGAAAGCTGGGTAATGGCTATAAAAAGACCAG
Amino acid length 390
Molecular weight 46.5
Isoelectric point (calc.) 5.1
Signal SEQ Predicted (N-terminus)
No. of transmembrane domain
NLS position (Columbia Univ. Bioinformatics Center) none
NES motif ( L-x(2,3)-[IVLMF]-x(2,3)-L-x-[LI]) LPRKFKRVLLL
Localization (YFP) ER; Golgi
Comments for localization
Effect of LMB on protein localization no change
Microscope used for observation DeltaVision

Image information
YFP 2 images) See all images

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