Gene name |
SPBC19G7.04 |
Gene ID |
18/F03 |
Gene synonyms/obsolete |
|
Gene product |
conserved hypothetical
protein; involved in transcriptional regulation; HMG box |
Entry clone |
Cloned |
ORF length (unspliced) |
1174 |
ORF length (spliced) |
1089 |
Entry clone length |
1174 |
No. of intron |
2 |
Sequence status |
Finished |
Sequence results |
68A:G / 1062A:G |
Comments |
|
Polymerase used for cloning |
Platinum Taq HiFi
(Invitrogen) |
Fwd primer name |
SPBC19G7.04.Fd |
Fwd primer SEQ |
AAAAAGCAGGCTCTCATATGCAAGTTTCAAACAGCAT |
Rev primer name |
SPBC19G7.04.Rv |
Rev primer SEQ |
AGAAAGCTGGGTACAAATCGCTTGAAGAGATA |
Amino acid length |
362 |
Molecular weight |
41.6 |
Isoelectric point (calc.) |
9.9 |
Signal SEQ |
|
No. of transmembrane domain |
|
NLS position (Columbia Univ.
Bioinformatics Center) |
none |
NES motif (
L-x(2,3)-[IVLMF]-x(2,3)-L-x-[LI]) |
none |
Localization (YFP) |
nucleus; spindle
microtubules? |
Comments for localization |
|
Effect of LMB on protein
localization |
no change |
Microscope used for
observation |
DeltaVision |