| Gene name |
SPAC3F10.09 |
| Gene ID |
18/F10 |
| Gene synonyms/obsolete |
|
| Gene product |
1-(5-phosphoribosyl)-5-[(5-phosphoribosylamino)methylideneamino]imidazole-4-carboxamide
isomerase activity; involved in histidine biosynthesis (4th
step) |
| Entry clone |
Cloned |
| ORF length (unspliced) |
1179 |
| ORF length (spliced) |
795 |
| Entry clone length |
1179 |
| No. of intron |
3 |
| Sequence status |
Finished |
| Sequence results |
263T:deletion /
864T:C |
| Comments |
|
| Polymerase used for cloning |
Platinum Taq HiFi
(Invitrogen) |
| Fwd primer name |
SPAC3F10.09.Fd |
| Fwd primer SEQ |
AAAAAGCAGGCTCTCATATGGAAAAATCGCAGCACAC |
| Rev primer name |
SPAC3F10.09.Rv |
| Rev primer SEQ |
AGAAAGCTGGGTAGTCTTGGAGAGGCAATGGA |
| Amino acid length |
264 |
| Molecular weight |
29.3 |
| Isoelectric point (calc.) |
5.6 |
| Signal SEQ |
|
| No. of transmembrane domain |
|
| NLS position (Columbia Univ.
Bioinformatics Center) |
none |
| NES motif (
L-x(2,3)-[IVLMF]-x(2,3)-L-x-[LI]) |
LCKGIDEDLVI |
| Localization (YFP) |
cytosol=nucleus |
| Comments for localization |
|
| Effect of LMB on protein
localization |
no change |
| Microscope used for
observation |
Confocal
|