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Schizosaccharomyces pombe Postgenome Database

Gene name SPAC56F8.12
Gene ID 18/G10
Gene synonyms/obsolete
Gene product sequence orphan; hypothetical protein
Entry clone Cloned
ORF length (unspliced) 1185
ORF length (spliced)
Entry clone length 1185
No. of intron 0
Sequence status Finished
Sequence results 55T:C / 958G:A
Comments
Polymerase used for cloning Platinum Taq HiFi (Invitrogen)
Fwd primer name SPAC56F8.12.Fd
Fwd primer SEQ AAAAAGCAGGCTCTCATATGATGAATGATTCACAAGT
Rev primer name SPAC56F8.12.Rv
Rev primer SEQ AGAAAGCTGGGTACTCCCGTACCTCACCCGAT
Amino acid length 394
Molecular weight 44.5
Isoelectric point (calc.) 7.1
Signal SEQ
No. of transmembrane domain 7
NLS position (Columbia Univ. Bioinformatics Center) 332
NES motif ( L-x(2,3)-[IVLMF]-x(2,3)-L-x-[LI]) none
Localization (YFP) Golgi
Comments for localization
Effect of LMB on protein localization no change
Microscope used for observation DeltaVision

Image information
YFP 2 images) See all images

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