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Schizosaccharomyces pombe Postgenome Database

Gene name SPAC1F7.07c
Gene ID 19/A08
Gene synonyms/obsolete fip1
Gene product iron permease
Entry clone Cloned
ORF length (unspliced) 1194
ORF length (spliced)
Entry clone length 1194
No. of intron 0
Sequence status Finished
Sequence results 342T:C
Comments
Polymerase used for cloning Platinum Taq HiFi (Invitrogen)
Fwd primer name SPAC1F7.07.Fd
Fwd primer SEQ AAAAAGCAGGCTCTCATATGGCAAAAGACGTCTTTTC
Rev primer name SPAC1F7.07.Rv
Rev primer SEQ AGAAAGCTGGGTAATGAGACTCACGAATTTCA
Amino acid length 397
Molecular weight 44.3
Isoelectric point (calc.) 6.5
Signal SEQ Predicted (N-terminus)
No. of transmembrane domain 7
NLS position (Columbia Univ. Bioinformatics Center) none
NES motif ( L-x(2,3)-[IVLMF]-x(2,3)-L-x-[LI]) none
Localization (YFP) ER; Golgi?
Comments for localization
Effect of LMB on protein localization no change
Microscope used for observation DeltaVision

Image information
YFP 1 images) See all images

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