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Schizosaccharomyces pombe Postgenome Database

Gene name SPBP8B7.10c
Gene ID 19/A11
Gene synonyms/obsolete
Gene product conserved hypothetical protein; possibly involved in protein synthesis ?????; coiled-coil?
Entry clone Cloned
ORF length (unspliced) 1194
ORF length (spliced) 1041
Entry clone length 1194
No. of intron 2
Sequence status Finished
Sequence results 100% match
Comments
Polymerase used for cloning Platinum Taq HiFi (Invitrogen)
Fwd primer name SPBP8B7.10.Fd
Fwd primer SEQ AAAAAGCAGGCTCTCATATGCAATCGTCTAGAAAAAT
Rev primer name SPBP8B7.10.Rv
Rev primer SEQ AGAAAGCTGGGTAATAAGTCAAGGGACGTCGT
Amino acid length 346
Molecular weight 40
Isoelectric point (calc.) 10.5
Signal SEQ
No. of transmembrane domain 1
NLS position (Columbia Univ. Bioinformatics Center) 323
NES motif ( L-x(2,3)-[IVLMF]-x(2,3)-L-x-[LI]) none
Localization (YFP) nucleolus>nucleus
Comments for localization
Effect of LMB on protein localization no change
Microscope used for observation Confocal

Image information
YFP 2 images) See all images

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