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Schizosaccharomyces pombe Postgenome Database

Gene name SPCC757.09c
Gene ID 19/B07
Gene synonyms/obsolete
Gene product RNA-binding protein; KH domain; involved in polyadenylation
Entry clone Cloned
ORF length (unspliced) 1197
ORF length (spliced)
Entry clone length 1197
No. of intron 0
Sequence status Finished
Sequence results 1162C:T
Comments
Polymerase used for cloning Platinum Taq HiFi (Invitrogen)
Fwd primer name SPCC757.09.Fd
Fwd primer SEQ AAAAAGCAGGCTCTCATATGGCTTACAATCACTTCAG
Rev primer name SPCC757.09.Rv
Rev primer SEQ AGAAAGCTGGGTAATGAGAACGACGATCTTTT
Amino acid length 398
Molecular weight 43.3
Isoelectric point (calc.) 7.6
Signal SEQ
No. of transmembrane domain
NLS position (Columbia Univ. Bioinformatics Center) none
NES motif ( L-x(2,3)-[IVLMF]-x(2,3)-L-x-[LI]) none
Localization (YFP) cytosol
Comments for localization
Effect of LMB on protein localization no change
Microscope used for observation DeltaVision

Image information
YFP 1 images) See all images

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