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Schizosaccharomyces pombe Postgenome Database

Gene name SPBC56F2.12
Gene ID 19/C11
Gene synonyms/obsolete
Gene product acetohydroxyacid reductoisomerase; ketol-acid reductoisomerase family; involved in valine biosynthesis; involved in isoleucine biosynthesis; possibly involved in mitochondrial DNA stability
Entry clone Cloned
ORF length (unspliced) 1215
ORF length (spliced)
Entry clone length 1215
No. of intron 0
Sequence status Finished
Sequence results 100% match
Comments
Polymerase used for cloning Platinum Taq HiFi (Invitrogen)
Fwd primer name SPBC56F2.12.Fd
Fwd primer SEQ AAAAAGCAGGCTCTCATATGTCTTTCCGTAATTCCTC
Rev primer name SPBC56F2.12.Rv
Rev primer SEQ AGAAAGCTGGGTAGTGCTTGTTGTGTTCAGGA
Amino acid length 404
Molecular weight 45.1
Isoelectric point (calc.) 9.9
Signal SEQ
No. of transmembrane domain
NLS position (Columbia Univ. Bioinformatics Center) none
NES motif ( L-x(2,3)-[IVLMF]-x(2,3)-L-x-[LI]) none
Localization (YFP) mitochondrion
Comments for localization bright signal
Effect of LMB on protein localization no change
Microscope used for observation DeltaVision

Image information
YFP 1 images) See all images

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