Gene name |
SPBC56F2.12 |
Gene ID |
19/C11 |
Gene synonyms/obsolete |
|
Gene product |
acetohydroxyacid
reductoisomerase; ketol-acid reductoisomerase family; involved
in valine biosynthesis; involved in isoleucine biosynthesis;
possibly involved in mitochondrial DNA stability |
Entry clone |
Cloned |
ORF length (unspliced) |
1215 |
ORF length (spliced) |
|
Entry clone length |
1215 |
No. of intron |
0 |
Sequence status |
Finished |
Sequence results |
100% match |
Comments |
|
Polymerase used for cloning |
Platinum Taq HiFi
(Invitrogen) |
Fwd primer name |
SPBC56F2.12.Fd |
Fwd primer SEQ |
AAAAAGCAGGCTCTCATATGTCTTTCCGTAATTCCTC |
Rev primer name |
SPBC56F2.12.Rv |
Rev primer SEQ |
AGAAAGCTGGGTAGTGCTTGTTGTGTTCAGGA |
Amino acid length |
404 |
Molecular weight |
45.1 |
Isoelectric point (calc.) |
9.9 |
Signal SEQ |
|
No. of transmembrane domain |
|
NLS position (Columbia Univ.
Bioinformatics Center) |
none |
NES motif (
L-x(2,3)-[IVLMF]-x(2,3)-L-x-[LI]) |
none |
Localization (YFP) |
mitochondrion |
Comments for localization |
bright signal |
Effect of LMB on protein
localization |
no change |
Microscope used for
observation |
DeltaVision |