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Schizosaccharomyces pombe Postgenome Database

Gene name SPCC777.07
Gene ID 19/D02
Gene synonyms/obsolete
Gene product glycosyl transferase family 15; similar to S. cerevisiae YOR099W and YKR061W and YJL139C and YDR483W and YBR205W and YBR199W and YPL053C; conserved fungal protein
Entry clone Cloned
ORF length (unspliced) 1217
ORF length (spliced) 1137
Entry clone length 1217
No. of intron 1
Sequence status Finished
Sequence results 100% match
Comments
Polymerase used for cloning Platinum Taq HiFi (Invitrogen)
Fwd primer name SPCC777.07.Fd
Fwd primer SEQ AAAAAGCAGGCTCTCATATGGGCATACCCAAATCATC
Rev primer name SPCC777.07.Rv
Rev primer SEQ AGAAAGCTGGGTAACTATTGATGTTTTCTAAC
Amino acid length 378
Molecular weight 44.4
Isoelectric point (calc.) 6.5
Signal SEQ
No. of transmembrane domain 1
NLS position (Columbia Univ. Bioinformatics Center) none
NES motif ( L-x(2,3)-[IVLMF]-x(2,3)-L-x-[LI]) none
Localization (YFP) ER
Comments for localization
Effect of LMB on protein localization no change
Microscope used for observation Confocal

Image information
YFP 1 images) See all images

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