Gene name |
SPBC646.06c |
Gene ID |
19/E02 |
Gene synonyms/obsolete |
|
Gene product |
glycosyl hydrolase
family 71; glucanase; similar to Penicillium purpurogenum
mutanase; no apparent Sc ortholog |
Entry clone |
Cloned |
ORF length (unspliced) |
1224 |
ORF length (spliced) |
|
Entry clone length |
1224 |
No. of intron |
0 |
Sequence status |
Finished |
Sequence results |
777T:C / 1176T:C |
Comments |
|
Polymerase used for cloning |
Platinum Taq HiFi
(Invitrogen) |
Fwd primer name |
SPBC646.06.Fd |
Fwd primer SEQ |
AAAAAGCAGGCTCTCATATGGCGTCGTTAACAACAGC |
Rev primer name |
SPBC646.06.Rv |
Rev primer SEQ |
AGAAAGCTGGGTACCCCTGAGCGATAACTGTC |
Amino acid length |
407 |
Molecular weight |
45 |
Isoelectric point (calc.) |
5.9 |
Signal SEQ |
|
No. of transmembrane domain |
|
NLS position (Columbia Univ.
Bioinformatics Center) |
none |
NES motif (
L-x(2,3)-[IVLMF]-x(2,3)-L-x-[LI]) |
LNLQFLLYLNL |
Localization (YFP) |
nucleus>cytosol |
Comments for localization |
|
Effect of LMB on protein
localization |
no change |
Microscope used for
observation |
Leica |