Chemical Genetics Laboratory
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Schizosaccharomyces pombe Postgenome Database

Gene name SPAC732.02c
Gene ID 19/E06
Gene synonyms/obsolete
Gene product fructose-2,6-bisphosphatase
Entry clone Cloned
ORF length (unspliced) 1227
ORF length (spliced)
Entry clone length 1227
No. of intron 0
Sequence status Finished
Sequence results 100% match
Comments
Polymerase used for cloning Platinum Taq HiFi (Invitrogen)
Fwd primer name SPAC732.02.Fd
Fwd primer SEQ AAAAAGCAGGCTCTCATATGAAAGACATTGAAGGACT
Rev primer name SPAC732.02.Rv
Rev primer SEQ AGAAAGCTGGGTAATGTTTGCCGCGTTGAGTA
Amino acid length 408
Molecular weight 46.9
Isoelectric point (calc.) 6.1
Signal SEQ Predicted (N-terminus)
No. of transmembrane domain
NLS position (Columbia Univ. Bioinformatics Center) none
NES motif ( L-x(2,3)-[IVLMF]-x(2,3)-L-x-[LI]) none
Localization (YFP) no apparent signal
Comments for localization
Effect of LMB on protein localization not determined
Microscope used for observation Confocal

Image information
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