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Schizosaccharomyces pombe Postgenome Database

Gene name SPAC10F6.13c
Gene ID 19/E10
Gene synonyms/obsolete
Gene product aspartate aminotransferase
Entry clone Cloned
ORF length (unspliced) 1230
ORF length (spliced)
Entry clone length 1230
No. of intron 0
Sequence status Finished
Sequence results 100% match
Comments
Polymerase used for cloning Platinum Taq HiFi (Invitrogen)
Fwd primer name SPAC10F6.13.Fd
Fwd primer SEQ AAAAAGCAGGCTCTCATATGTCAGACTACGGGTTTGC
Rev primer name SPAC10F6.13.Rv
Rev primer SEQ AGAAAGCTGGGTAAGGCAACTCGCGAACAGCA
Amino acid length 409
Molecular weight 46.1
Isoelectric point (calc.) 7.6
Signal SEQ
No. of transmembrane domain
NLS position (Columbia Univ. Bioinformatics Center) none
NES motif ( L-x(2,3)-[IVLMF]-x(2,3)-L-x-[LI]) none
Localization (YFP) cytosol=nucleus
Comments for localization
Effect of LMB on protein localization no change
Microscope used for observation Confocal

Image information
YFP 1 images) See all images

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