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Schizosaccharomyces pombe Postgenome Database

Gene name SPBC428.15
Gene ID 19/F01
Gene synonyms/obsolete
Gene product conserved hypothetical; GTP-binding protein; OBG family
Entry clone Cloned
ORF length (unspliced) 1230
ORF length (spliced)
Entry clone length 1230
No. of intron 0
Sequence status Finished
Sequence results 100% match
Comments
Polymerase used for cloning Platinum Taq HiFi (Invitrogen)
Fwd primer name SPBC428.15.Fd
Fwd primer SEQ AAAAAGCAGGCTCTCATATGGGTAGAGACATTTTAAT
Rev primer name SPBC428.15.Rv
Rev primer SEQ AGAAAGCTGGGTAAGCTTCAGCTTTCCGCAAT
Amino acid length 409
Molecular weight 44.4
Isoelectric point (calc.) 7.2
Signal SEQ
No. of transmembrane domain
NLS position (Columbia Univ. Bioinformatics Center) none
NES motif ( L-x(2,3)-[IVLMF]-x(2,3)-L-x-[LI]) LAKAMELLNL
Localization (YFP) cytosol=nucleus
Comments for localization
Effect of LMB on protein localization no change
Microscope used for observation DeltaVision

Image information
YFP 1 images) See all images

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