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Schizosaccharomyces pombe Postgenome Database

Gene name SPAC56F8.16
Gene ID 19/H01
Gene synonyms/obsolete esc1
Gene product transcription factor; involved in sexual differentiation; basic helix-loop-helix (bHLH); DNA-binding protein
Entry clone Cloned#
ORF length (unspliced) 1242
ORF length (spliced)
Entry clone length 1242
No. of intron 0
Sequence status Finished
Sequence results 326A:T/ 341C:T/ 929A:T
Comments
Polymerase used for cloning Pyrobest DNA Pol (TaKaRa)
Fwd primer name SPAC56F8.16.Fd
Fwd primer SEQ AAAAAGCAGGCTCTCATATGTCTTCATACGCTCTTCC
Rev primer name SPAC56F8.16.Rv
Rev primer SEQ AGAAAGCTGGGTACGTTCCCTTAGTCACCTCC
Amino acid length 413
Molecular weight 44.7
Isoelectric point (calc.) 9.3
Signal SEQ
No. of transmembrane domain
NLS position (Columbia Univ. Bioinformatics Center) 343
NES motif ( L-x(2,3)-[IVLMF]-x(2,3)-L-x-[LI]) LFDDLKDALPL
Localization (YFP) nucleus; nuclear dots
Comments for localization except nucleolus
Effect of LMB on protein localization no change
Microscope used for observation DeltaVision

Image information
YFP 2 images) See all images

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