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Schizosaccharomyces pombe Postgenome Database

Gene name SPCC965.12
Gene ID 20/A02
Gene synonyms/obsolete
Gene product dipeptidase; no apparent Sc ortholog; non-essential
Entry clone Cloned
ORF length (unspliced) 1251
ORF length (spliced)
Entry clone length 1251
No. of intron 0
Sequence status Finished
Sequence results 41A:G
Comments
Polymerase used for cloning Platinum Taq HiFi (Invitrogen)
Fwd primer name SPCC965.12.Fd
Fwd primer SEQ AAAAAGCAGGCTCTCATATGACGGTTGATCTTAGGGA
Rev primer name SPCC965.12.Rv
Rev primer SEQ AGAAAGCTGGGTATTTCTCAATGTTCAAAGGC
Amino acid length 416
Molecular weight 47.4
Isoelectric point (calc.) 5.9
Signal SEQ
No. of transmembrane domain
NLS position (Columbia Univ. Bioinformatics Center) none
NES motif ( L-x(2,3)-[IVLMF]-x(2,3)-L-x-[LI]) LRVQLRNKLQL
Localization (YFP) cytosol=nucleus
Comments for localization
Effect of LMB on protein localization no change
Microscope used for observation DeltaVision

Image information
YFP 1 images) See all images

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