Chemical Genetics Laboratory
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Schizosaccharomyces pombe Postgenome Database

Gene name SPACUNK12.02c
Gene ID 20/A06
Gene synonyms/obsolete cmk1
Gene product serine/threonine protein kinase; calmodulin kinase I homolog; CaM-dependent protein kinase; cell cycle-dependent expression; predicted regulatory site (Thr-192)
Entry clone Cloned
ORF length (unspliced) 1256
ORF length (spliced) 1008
Entry clone length 1256
No. of intron 4
Sequence status Finished
Sequence results 37A:G / 246A:G / 465T:C / 1234A:G
Comments
Polymerase used for cloning Platinum Taq HiFi (Invitrogen)
Fwd primer name SPACUNK12.02.Fd
Fwd primer SEQ AAAAAGCAGGCTCTCATATGCAGCAAACATACAAACC
Rev primer name SPACUNK12.02.Rv
Rev primer SEQ AGAAAGCTGGGTAATGCTTATTCTCAAGCTTT
Amino acid length 335
Molecular weight 38.1
Isoelectric point (calc.) 9.1
Signal SEQ
No. of transmembrane domain
NLS position (Columbia Univ. Bioinformatics Center) none
NES motif ( L-x(2,3)-[IVLMF]-x(2,3)-L-x-[LI]) none
Localization (YFP) cytosol=nucleus
Comments for localization
Effect of LMB on protein localization no change
Microscope used for observation DeltaVision

Image information
YFP 1 images) See all images

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