Gene name |
SPBC19G7.15 |
Gene ID |
20/A07 |
Gene synonyms/obsolete |
|
Gene product |
nucleoporin; nuclear
pore complex |
Entry clone |
Cloned in 2006
trial |
ORF length (unspliced) |
1256 |
ORF length (spliced) |
1212 |
Entry clone length |
1256 |
No. of intron |
1 |
Sequence status |
Finished |
Sequence results |
100% match |
Comments |
|
Polymerase used for cloning |
Pyrobest DNA Pol
(TaKaRa) |
Fwd primer name |
SPBC19G7.15.Fd |
Fwd primer SEQ |
AAAAAGCAGGCTCTCATATGGCATTTTCTTTTGGTAT |
Rev primer name |
SPBC19G7.15.Rv |
Rev primer SEQ |
AGAAAGCTGGGTATTTTTGTGATTGAGGAACG |
Amino acid length |
403 |
Molecular weight |
44.4 |
Isoelectric point (calc.) |
10 |
Signal SEQ |
|
No. of transmembrane domain |
|
NLS position (Columbia Univ.
Bioinformatics Center) |
none |
NES motif (
L-x(2,3)-[IVLMF]-x(2,3)-L-x-[LI]) |
LSERILRLAI |
Localization (YFP) |
nuclear envelope;
nucleus>cytosol |
Comments for localization |
|
Effect of LMB on protein
localization |
no change |
Microscope used for
observation |
DeltaVision |