| Gene name |
SPBC1289.11 |
| Gene ID |
20/B01 |
| Gene synonyms/obsolete |
spf38; cwf17 |
| Gene product |
small nuclear
ribonucleoprotein (snRNP) U5 particle component; WD repeat
protein; complexed with Cdc5p; involved in mRNA splicing; no
apparent Sc ortholog |
| Entry clone |
Cloned |
| ORF length (unspliced) |
1257 |
| ORF length (spliced) |
1023 |
| Entry clone length |
1257 |
| No. of intron |
5 |
| Sequence status |
Finished |
| Sequence results |
553A:G |
| Comments |
|
| Polymerase used for cloning |
Platinum Taq HiFi
(Invitrogen) |
| Fwd primer name |
SPBC1289.11.Fd |
| Fwd primer SEQ |
AAAAAGCAGGCTCTCATATGGATAAACGAAAGGAATC |
| Rev primer name |
SPBC1289.11.Rv |
| Rev primer SEQ |
AGAAAGCTGGGTAATTCAATTCCCCAAGAAAT |
| Amino acid length |
340 |
| Molecular weight |
37.4 |
| Isoelectric point (calc.) |
7.4 |
| Signal SEQ |
|
| No. of transmembrane domain |
|
| NLS position (Columbia Univ.
Bioinformatics Center) |
none |
| NES motif (
L-x(2,3)-[IVLMF]-x(2,3)-L-x-[LI]) |
none |
| Localization (YFP) |
nucleus |
| Comments for localization |
|
| Effect of LMB on protein
localization |
no change |
| Microscope used for
observation |
DeltaVision |