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Schizosaccharomyces pombe Postgenome Database

Gene name SPBC16H5.09c
Gene ID 20/B07
Gene synonyms/obsolete
Gene product glycosyl transferase family 15; similar to S. pombe SPCC777.07 and SPBC19C7.12c; similar to S. cerevisiae YOR099W and YKR061W and YJL139C and YDR483W and YBR205W and YBR199W and YPL053C; conserved fungal protein
Entry clone Cloned
ORF length (unspliced) 1262
ORF length (spliced) 1119
Entry clone length 1262
No. of intron 3
Sequence status Finished
Sequence results 100% match
Comments
Polymerase used for cloning Platinum Taq HiFi (Invitrogen)
Fwd primer name SPBC16H5.09.Fd
Fwd primer SEQ AAAAAGCAGGCTCTCATATGCGGATATCAAGATTGCT
Rev primer name SPBC16H5.09.Rv
Rev primer SEQ AGAAAGCTGGGTATAATTCATCGTAGTCTTCG
Amino acid length 372
Molecular weight 44.3
Isoelectric point (calc.) 6.9
Signal SEQ Predicted (N-terminus)
No. of transmembrane domain 1
NLS position (Columbia Univ. Bioinformatics Center) none
NES motif ( L-x(2,3)-[IVLMF]-x(2,3)-L-x-[LI]) LLGFVILFI
Localization (YFP) ER
Comments for localization
Effect of LMB on protein localization no change
Microscope used for observation DeltaVision

Image information
YFP 1 images) See all images

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