| Gene name |
SPCC1919.03c |
| Gene ID |
20/C01 |
| Gene synonyms/obsolete |
|
| Gene product |
5'-amp-activated
protein kinase beta subunit; involved in the regulation of
fatty acid synthesis by the phosphorylation of acetyl-CoA
carboxylase; involved in the regulation of carbohydrate
metabolism; N-myristoylated (ISS) |
| Entry clone |
Cloned |
| ORF length (unspliced) |
1264 |
| ORF length (spliced) |
897 |
| Entry clone length |
1264 |
| No. of intron |
4 |
| Sequence status |
Finished |
| Sequence results |
1080T:G |
| Comments |
|
| Polymerase used for cloning |
Platinum Taq HiFi
(Invitrogen) |
| Fwd primer name |
SPCC1919.03.Fd |
| Fwd primer SEQ |
AAAAAGCAGGCTCTCATATGGGAAATGTTCAATCTCA |
| Rev primer name |
SPCC1919.03.Rv |
| Rev primer SEQ |
AGAAAGCTGGGTAGACATCAAAATTTTTAAAC |
| Amino acid length |
298 |
| Molecular weight |
32.9 |
| Isoelectric point (calc.) |
6.3 |
| Signal SEQ |
|
| No. of transmembrane domain |
|
| NLS position (Columbia Univ.
Bioinformatics Center) |
none |
| NES motif (
L-x(2,3)-[IVLMF]-x(2,3)-L-x-[LI]) |
none |
| Localization (YFP) |
ambiguous structure;
cytosol |
| Comments for localization |
|
| Effect of LMB on protein
localization |
no change |
| Microscope used for
observation |
DeltaVision |