Gene name |
SPAC3A12.11c |
Gene ID |
20/C12 |
Gene synonyms/obsolete |
cwf2; prp3 |
Gene product |
involved in mRNA
splicing; zinc finger protein; zf-CCCH type; RNA-binding
protein; rrm RNA recognition motif; RNP-containing protein;
40S snRNP-containing complex; nineteen complex (Ntc);
complexed with Cdc5p; interacts physically with Cdc5p;
interacts physically with Cwf8p; mutant (prp3-1) accumulates
unspliced mRNAs |
Entry clone |
Cloned |
ORF length (unspliced) |
1270 |
ORF length (spliced) |
1167 |
Entry clone length |
1270 |
No. of intron |
1 |
Sequence status |
Finished |
Sequence results |
676T:C |
Comments |
|
Polymerase used for cloning |
Platinum Taq HiFi
(Invitrogen) |
Fwd primer name |
SPAC3A12.11.Fd |
Fwd primer SEQ |
AAAAAGCAGGCTCTCATATGTCAGAAAATGGGTTAGA |
Rev primer name |
SPAC3A12.11.Rv |
Rev primer SEQ |
AGAAAGCTGGGTAGTCTTCATCACTATCGTAA |
Amino acid length |
388 |
Molecular weight |
44.2 |
Isoelectric point (calc.) |
5.4 |
Signal SEQ |
|
No. of transmembrane domain |
|
NLS position (Columbia Univ.
Bioinformatics Center) |
37 |
NES motif (
L-x(2,3)-[IVLMF]-x(2,3)-L-x-[LI]) |
none |
Localization (YFP) |
nucleus |
Comments for localization |
|
Effect of LMB on protein
localization |
no change |
Microscope used for
observation |
DeltaVision |