Chemical Genetics Laboratory
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Schizosaccharomyces pombe Postgenome Database

Gene name SPAC6B12.08
Gene ID 20/D10
Gene synonyms/obsolete
Gene product zinc finger protein; zf-C2H2 type; DNAJ domain protein
Entry clone Cloned
ORF length (unspliced) 1277
ORF length (spliced) 1143
Entry clone length 1277
No. of intron 1
Sequence status Finished
Sequence results 100% match
Comments
Polymerase used for cloning Platinum Taq HiFi (Invitrogen)
Fwd primer name SPAC6B12.08.Fd
Fwd primer SEQ AAAAAGCAGGCTCTCATATGAATAACCCTTTTAAGGA
Rev primer name SPAC6B12.08.Rv
Rev primer SEQ AGAAAGCTGGGTAATCTTGATCCAACTTATCG
Amino acid length 380
Molecular weight 45.1
Isoelectric point (calc.) 4.9
Signal SEQ
No. of transmembrane domain
NLS position (Columbia Univ. Bioinformatics Center) none
NES motif ( L-x(2,3)-[IVLMF]-x(2,3)-L-x-[LI]) none
Localization (YFP) ER
Comments for localization
Effect of LMB on protein localization no change
Microscope used for observation DeltaVision

Image information
YFP 1 images) See all images

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